%0 Journal Article
%T Fruit set and plant regeneration in cassava following interspecific pollination with castor bean
%A A.K.
%A B.
%A Baguma
%A Buttibwa
%A Ceballos
%A Eyokia
%A H.
%A J.K.
%A Kawuki
%A Lentini
%A M.
%A Mukasa
%A Nalela
%A Oshaba
%A P.
%A R.
%A S.B.
%A Tugume
%A Y.
%A Z. & Baguma
%J African Crop Science Journal
%D 2019
%X The increasing demand for cassava ( Manihot esculenta Crantz) for food and non-food uses in the tropics necessitates that its breeding for increased root productivity be made faster. The characteristic long breeding cycle and heterozygous nature of this crop, pose a major obstacle to its rapid genetic improvement. This study aimed at inter-pollinating cassava with castor bean ( Ricinus communis ), with a purpose of inducing and regenerating cassava doubled haploids (DHs). A total of 3,349 flowers from twelve elite cassava varieties were inter-pollinated with caster bean. A total of 803 fruits were harvested for early embryo rescue and/or ovule culture. Of these, three were dissected to obtain seven unique embryos, while 800 were dissected to obtain 1312 young ovules, all of which were cultured in vitro. Overall, 82 (6.25%) of the cultured ovules formed callus that originated from the embryosac region, which is haploid. Four out of seven rescued embryos (57.1%) regenerated into plantlets. Ploidy analyses of 24 samples using flow cytometry revealed that 23 of the analysed samples were diploid. However, one callus sample was anueploid. Only one sample had an exceptionally high level of homozygosity ( 84.2%). These findings lay a foundation for future research aimed at induction of haploids in cassava. La demande croissante de manioc ( Manihot esculenta Crantz ) ¨¤ usage alimentaire et non alimentaire dans les tropiques n¨¦cessite que sa reproduction pour une productivit¨¦ accrue des racines soit faite plus rapidement. Le long cycle de reproduction et le caract¨¨re h¨¦t¨¦rozygote de cette plante constituent un obstacle majeur dans la rapidit¨¦ de son am¨¦lioration g¨¦n¨¦tique. Cette ¨¦tude visait ¨¤ inter-polliniser le manioc avec le haricot ( Ricinus communis ), dans le but d¡¯induire et de r¨¦g¨¦n¨¦rer le manioc d¡¯haplo£¿des doubl¨¦ (HD). Un total de 3 349 fleurs de douze ¨¦lites vari¨¦t¨¦s de manioc ont ¨¦t¨¦ inter-pollinis¨¦es avec le haricot. Un total de 803 fruits ont ¨¦t¨¦ r¨¦colt¨¦s pour les embryons pr¨¦matur¨¦s qui etaient sauves et / ou la culture d¡¯ovules . Parmi ceux-ci, trois ont ¨¦t¨¦ diss¨¦qu¨¦s pour obtenir sept embryons uniques , tandis que 800 ont ¨¦t¨¦ diss¨¦qu¨¦s pour obtenir 1312 jeunes ovules, qui ont tous ¨¦t¨¦ cultiv¨¦s in vitro . Un total de 82 (6,25%) des ovules en culture ont form¨¦ des cals provenant de la r¨¦gion embryonnaire , qui est haplo£¿de. Quatre parmi sept embryons sauv¨¦s (57,1%) se sont r¨¦g¨¦n¨¦r¨¦s en plantules. Les analyses de plo£¿die de 24 ¨¦chantillons par cytom¨¦trie en flux ont r¨¦v¨¦l¨¦ que 23 des ¨¦chantillons analys¨¦s ¨¦taient diplo£¿des.Cependant, un ¨¦chantillon
%U http://www.bioline.org.br/abstract?id=cs19008&lang=en