%0 Journal Article
%T Diabetes mellitus increased integrins gene expression in rat endometrium at the time of embryo implantation
%A Abbas
%A Bakhteyari
%A Eskandari
%A Fatemah Sadat
%A Hosseiny
%A Matinfar
%A Mohammad & Aboutorabi
%A Mostafavi
%A Nahid
%A Nikpour
%A Parvaneh
%A Roshanak
%A Yasaman
%A Zarrin
%A Zeinab Sadat
%J International Journal of Reproductive BioMedicine
%D 2019
%X Background: Diabetes mellitus deeply changes the genes expression of integrin ( Itg) subunits in several cells and tissues such as monocytes, arterial endothelium, kidney glomerular cells, retina. Furthermore, hyperglycemia could impress and reduce the rate of successful assisted as well as non-assisted pregnancy. Endometrium undergoes thorough changes in normal menstrual cycle and the question is: What happens in the endometrium under diabetic condition? Objective: The aim of the current study was to investigate the endometrial gene expression of ¦Á3, ¦Á4, ¦Áv, Itg ¦Â1 and ¦Â3 subunits in diabetic rat models at the time of embryo implantation. Materials and Methods: Twenty-eight rats were randomly divided into 4 groups: control group, diabetic group, pioglitazone-treated group, and metformin-treated group. Real-time PCR was performed to determine changes in the expression of Itg ¦Á3, ¦Á4, ¦Áv, ¦Â1, and ¦Â3 genes in rat¡¯s endometrium. Results: The expression of all Itg subunits increased significantly in diabetic rats¡¯ endometrium compared with control group. Treatment with pioglitazone significantly reduced the level of Itg subunits gene expression compared with diabetic rats. While metformin had a different effect on ¦Á3 and ¦Á4 and elevated these two subunits gene expression. Conclusion: Diabetes mellitus significantly increased the expression of studied Itg subunits, therefore untreated diabetes could be potentially assumed as one of the preliminary elements in embryo implantation failure
%U http://www.bioline.org.br/abstract?id=rm19042&lang=en