%0 Journal Article
%T Gold immunochromatographic assay for kitasamycin and josamycin residues screening in milk and egg samples
%A Gang Cui
%A Hua Kuang
%A Lingling Guo
%A Liqiang Liu
%A Shufeng Ma
%A Xiaoling Wu
%J Food and Agricultural Immunology
%D 2019
%R https://doi.org/10.1080/09540105.2019.1677567
%X ABSTRACT In this study, a kitasamycin (KIT) hapten was prepared through the o-carboxymethoxylamine method and used to produce a monoclonal antibody against KIT and josamycin (JOS). The half inhibition concentration for an indirect competitive enzyme-linked immunosorbent assay was 1.49 and 2.67£¿ng/mL, respectively. Based on this antibody, a gold immunochromatographic assay (GICA) was developed for the detection of KIT and JOS residues in milk and egg samples. A hand-held strip scanner was used for quantitative analysis, with limits of detection for KIT and JOS of 1.51 and 1.91£¿¦Ìg/L in milk, and 3.0 and 2.72£¿¦Ìg/kg in eggs. The proposed GICA was validated by LC¨CMS/MS, which indicated that the GICA method would be a useful tool for the on-site detection of KIT and JOS residues in milk and egg samples
%U https://www.tandfonline.com/doi/full/10.1080/09540105.2019.1677567