%0 Journal Article
%T Development of a noncompetitive idiometric nanobodies phage immumoassay for the determination of fumonisin B1
%A Bruce D. Hammock
%A Chan Zhong
%A Guo-ping Wu
%A Jie-xian Dong
%A Mei Shu
%A Wen-jun Wang
%A Yang Xu
%J Food and Agricultural Immunology
%D 2019
%R https://doi.org/10.1080/09540105.2019.1604637
%X ABSTRACT Here we demonstrate a noncompetitive idiometric nanobodies phage immunoassay for the determination of FB1, utilizing two types of anti-idiotypic nanobody, that is specific for different epitopes within the hypervariable region of the primary mAb. Three alphatype anti-idiotypic nanobodies were obtained from a naive nanobodies phage-display library. A noncompetitive idiometric immunoassay was established with a combination of betatype anti-idiotypic nanobody and phage-displayed alphatype anti-idiotypic nanobody. The half-maximal saturation of the signal value (SC50) was 0.68£¿ng/mL, with the limit of detection (LOD) was 0.19£¿ng/mL. When the noncompetitive assay was compared to the competitive ELISA (LOD£¿=£¿3.41£¿ng/mL), an over 17-fold improvement in sensitivity was observed. A correlation (R2) was 0.988 between the data of the noncompetitive assay and LC¨CMS/MS for the determination of FB1 in cereal samples. The noncompetitive idiometric immunoassay would have a broad utility for monitoring small molecules in food and environment. GRAPHICAL ABSTRAC
%U https://www.tandfonline.com/doi/full/10.1080/09540105.2019.1604637