%0 Journal Article
%T Determination of 3.5-dinitro-N¡¯-(5-nitrofurfurylidene) salicylic acid hydrazide in fish using immunochromatographic strip tests
%A Dejun Ji
%A Hengpeng Wang
%A Lixia Fu
%A Peng Wu
%A Wei Zhang
%A Xiangren Meng
%J Food and Agricultural Immunology
%D 2019
%R https://doi.org/10.1080/09540105.2019.1600473
%X ABSTRACT The nitrofurans were recently banned from use in livestock production due to their potential carcinogenicity in humans. Therefore, the screening of foods from animal origins for nitrofurans became mandatory. A rapid, specific and sensitive method based on immunochromatographic strip test has been developed for the determination of 3.5-dinitro-N¡¯-(5-nitrofurfurylidene) salicylic acid hydrazide (DNSH) residues in fish. Fish samples were prepared by ultrasonic bath extraction, centrifugation, filtration, drying and res-suspension. The antigen (DNSH-glutaraldehyde-ovalbumin) and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane as the test and control line, respectively. Under optimal conditions, the DNSH cut-off limits for the test strips were determined as 10£¿ng/mL in 0.01 M phosphate buffered saline and 25£¿ng/mL in fish samples, with results obtained within 5min. Our data suggests this immunochromatographic assay can be used for the sensitive, rapid, and specific on-site screening of DNSH in fish products
%U https://www.tandfonline.com/doi/full/10.1080/09540105.2019.1600473