%0 Journal Article
%T Synergistic effects of deuterium depleted water and Mentha longifolia L. essential oils on sepsis-induced liver injuries through regulation of cyclooxygenase-2
%A Atoosa Vaziri
%A Azadeh Rasooli
%A Faezeh Fatemi
%A Kambiz Akbarzadeh
%A Maria Foroutanrad
%A Mohammad Reza Mohammadi Malayeri
%A Reza Hajihosseini
%A Salome Dini
%J Pharmaceutical Biology
%D 2019
%R https://doi.org/10.1080/13880209.2018.1563622
%X Abstract Context: Mentha longifolia L. (Lamiaceae), a traditional medicinal herb, has been highly valued for exhibiting antimicrobial, antioxidant and antispasmodic properties. Objective: For the first time, the synergetic anti-inflammatory effects of deuterium depleted water (DDW) and M. longifolia essential oils (ML) were investigated in experimental sepsis. Materials and methods: Fifty Wistar rats were divided into 5 groups (n£¿=£¿10): negative control (laparotomy), CLP, treatment groups including the combination of DDWs (15 and 30£¿ppm) and ML (100£¿mg/kg b.w) and indomethacin. At 24£¿h after CLP induction, lipid peroxidation (LP), glutathione (GSH), glutathione in S-transferases (GST), ferric reducing ability of plasma (FRAP), myeloperoxidase (MPO), prostaglandin E2 (PGE2), and COX-2 expression were determined in the plasma and liver tissues. Results: Compared with the CLP group, the administration of DDWs and ML significantly (p£¿<£¿0.05) prevented the increase of LP, COX-2 and PGE2 levels and liver enzymes. Additionally, the decreased levels of FRAP and GSH induced by sepsis were remarkably (p£¿<£¿0.05) risen by the administration of DDWs and ML in comparison to the CLP group. However, no significant (p£¿>£¿0.05) differences were observed regarding GST, ALP and bilirubin levels. Our results also proved the synergistic anti-inflammatory activities of the DDWs and ML. The anti-inflammatory effects of the DDWs and ML were confirmed by histopathological studies. Discussion and conclusions: The combination of DDWs and ML exerted synergistic anti-inflammatory activity against CLP-induced sepsis possibly through modulating oxidative stress/antioxidant parameters
%U https://www.tandfonline.com/doi/full/10.1080/13880209.2018.1563622