%0 Journal Article
%T Effects of steroidal saponins extract from Ophiopogon japonicus root ameliorates doxorubicin-induced chronic heart failure by inhibiting oxidative stress and inflammatory response
%A Akira Miyamoto
%A Chaoquan Liu
%A HongTao Wang
%A Shengji Zhao
%A Weimin Zheng
%A Xuekai Zhao
%A Zhongwei Wu
%J Pharmaceutical Biology
%D 2019
%R https://doi.org/10.1080/13880209.2019.1577467
%X Abstract Context: Ophiopogonis Radix, the root of Ophiopogon japonicus (Thunb.) Ker-Gawl (Liliaceae), is a Traditional Chinese Medicine, which has been investigated to possess effective treatment of cardiovascular diseases. Objective: This study evaluates the cardioprotective effects of steroidal saponins extract from Ophiopogon japonicus (SOJ) root against doxorubicin-induced chronic heart failure (CHF) through the amelioration of oxidative stress and inflammation. Materials and methods: A Sprague-Dawley rat model of CHF was established by intraperitoneally injected with DOX. All rats were randomly divided into four groups: Control group, CHF group, CHF£¿+£¿SOJ (100£¿mg/kg) treatment group, SOJ (100£¿mg/kg) treatment group (n£¿=£¿8/group). After six weeks administration, biometric and echocardiography were measured. The levels of biochemical parameters were measured using commercial kits. Results: The values of LVESP, +dP/dtmax, ¨CdP/dtmax, EF and FS increased to 116.20£¿¡À£¿1.68£¿mmHg, 2978.71£¿¡À£¿168.26£¿mmHg/s, 3452.61£¿¡À£¿286.09£¿mmHg/s, 68.26£¿¡À£¿5.28% and 31.97£¿¡À£¿3.79%, respectively; the values of LVEDP, LVESD and LVEDD decreased to 8.85£¿¡À£¿0.84£¿mmHg, 8.39£¿¡À£¿0.45£¿mm and 12.36£¿¡À£¿0.87£¿mm in CHF£¿+£¿SOJ group. In addition, the levels of IL-6, TNF-¦Á and IL-1¦Â decreased to 154.41£¿¡À£¿7.72£¿pg/mg protein, 110.02£¿¡À£¿6.96£¿pg/mg protein and 39.39£¿¡À£¿5.27£¿pg/mg protein, respectively; the relative activity of p38 MAPK decreased to 2.60£¿¡À£¿0.40 in CHF£¿+£¿SOJ group. Furthermore, the activities of SOD, CAT and GSH-Px increased to 268.77£¿¡À£¿6.20£¿U/mg protein, 13.68£¿¡À£¿0.68£¿U/mg protein and 316.90£¿¡À£¿8.08 ¦Ìmol/mg protein, and the content of MDA decreased to 4.03£¿¡À£¿0.43£¿nmol/mg protein in CHF£¿+£¿SOJ group. Conclusions: SOJ exerts the cardioprotective effect against DOX-induced CHF through suppressing inflammatory and oxidative stress. These results provide evidence that SOJ might be an effective treatment for CHF
%U https://www.tandfonline.com/doi/full/10.1080/13880209.2019.1577467