%0 Journal Article
%T Expression of BTK/p-BTK is different between CD5+ and CD5- B lymphocytes from Autoimmune Hemolytic Anemia/Evans syndromes
%A Hong Liu
%A Huaquan Wang
%A Limin Xing
%A Manjun Zhao
%A Ningning Duan
%A Yi Wang
%A Yingying Qu
%A Zonghong Shao
%J Hematology
%D 2019
%R https://doi.org/10.1080/16078454.2019.1652005
%X ABSTRACT Objective: To explore the activity of B subsets from Autoimmune Hemolytic Anemia/Evans syndrome (AIHA/ES) patients. Methods: The expression of Bruton¡¯s tyrosine kinase (BTK) and phosphorylated BTK (p-BTK) on CD5+CD19+B and CD5-CD19+B lymphocytes were detected using flow cytometry in AIHA/ES patients with different disease states, healthy controls (HCs) and chronic lymphocytic leukemia (CLL) patients. The correlations of expressed BTK and p-BTK with clinical variables were analyzed. Results: Thirty six AIHA/ES patients (16 hemolytic, 20 remission), 11 CLL patients, and 15 HCs were enrolled. The expression levels of BTK and p-BTK on CD5+B lymphocytes in AIHA/ES patients were higher than those in HCs and CLL patients. The latter two groups had no significant difference, and were positively correlated with the quantity of IgE. The ratio of p-BTK to BTK on CD5+B lymphocytes of the hemolytic and remission groups was obviously higher than that on CD5-B lymphocytes (74.62£¿¡À£¿6.42% and 29.63£¿¡À£¿10.19%, respectively; P£¿=£¿0.001 versus 77.95£¿¡À£¿9.57% and 26.29£¿¡À£¿6.86%, respectively; P£¿=£¿0.006). The ratio of p-BTK to BTK on CD5+B lymphocytes (54.89£¿¡À£¿9.56%) and CD5-B lymphocytes (30.86£¿¡À£¿12.47%) did not differ significantly in HCs (P£¿=£¿0.109). BTK did not differ significantly between CD5+ and CD5-B lymphocytes in AIHA/ES, but p-BTK on CD5+B lymphocytes was significantly higher than that on CD5-B lymphocytes in AIHA/ES patients. Conclusions: CD5+B lymphocytes are the major B subtype that is activated in AIHA/ES patients and it positively correlates with IgE
%U https://www.tandfonline.com/doi/full/10.1080/16078454.2019.1652005