%0 Journal Article
%T A Rapid and Effective Colony PCR Procedure for Screening Transformants in Several Common Mushrooms
%A Aimin Ma
%A Danyun Xu
%A Dongmei Liu
%A Liguo Chen
%A Lisheng Zheng
%A Xueyan Sun
%A Yuanyuan Wang
%A Yue Chen
%J Mycobiology
%D 2019
%R https://doi.org/10.1080/12298093.2019.1628523
%X Abstract In the post-genomic era, gene function analysis has attracted much attention. Transformation is often needed to investigate gene function. In this study, an easy, rapid, reliable, and cost-effective colony polymerase chain reaction (PCR) method for screening mushroom transformants was developed: picking up a suitable amount of transformant¡¯s tissue (1¨C10£¿¦Ìg) to 20£¿¦Ìl 0.25% Lywallzyme solution, and vortexing for 10£¿s followed by incubation at 34£¿¡ãC for 15£¿min. Finally, 2£¿¦Ìl of the suspension was used as templates to perform PCR and single target bands were successfully amplified from respective transformants of Tremella fuciformis, Pleurotus ostreatus, and Pleurotus tuber-regium. This procedure could be widely employed for screening transformants in mushroom transformation experiments
%U https://www.tandfonline.com/doi/full/10.1080/12298093.2019.1628523