%0 Journal Article
%T Heat shock protein expression and autophagy after incomplete thermal ablation and their correlation
%A Fen Chen
%A Guo Tian
%A Haiwei Bao
%A Haiyang Xie
%A Tianan Jiang
%J International Journal of Hyperthermia
%D 2019
%R https://doi.org/10.1080/02656736.2018.1536285
%X Abstract Objective: To establish a model of incomplete ablation in nude mice with hepatocellular carcinoma (HCC) and to evaluate heat shock protein (HSP) expression and autophagy and their correlation. Materials and methods: In the first stage, 12 nude mice with HCC were randomly divided into two groups (n£¿=£¿6). A sham puncture operation was performed for one group, and palliative laser ablation was performed for the other group. All mice were sacrificed after 18£¿h, and HSP expression, autophagy, and apoptosis were assessed. In the second stage, 16 nude mice with HCC were randomly divided into two groups (n£¿=£¿8). One group was given an HSP90 inhibitor before the operation, and the other group was given dimethyl sulfoxide (DMSO) as a control. HSP expression, autophagy and apoptosis were assessed for the two groups after palliative laser ablation. Results: In the incomplete ablation model, using nude mice with HCC, HSP90, HSP70, and HSP27 expression was up-regulated, Akt and mTOR phosphorylation was enhanced, autophagy was decreased, and apoptosis was increased. After administration of the HSP90 inhibitor, HSP90, P-Akt, and P-mTOR expression was decreased, autophagy was increased, and apoptosis was further increased. Conclusion: Autophagy was decreased in the incomplete ablation model and might be inversely correlated with HSP expression. It is suggested that the HSP90/Akt/mTOR pathway is involved in signal transmission between autophagy and HSPs
%U https://www.tandfonline.com/doi/full/10.1080/02656736.2018.1536285