%0 Journal Article
%T Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk
%A Chen Liu
%A Shimin Peng
%A Wenxiao Jiang
%A Xiaona Chen
%A Xin Zou
%A Yuebin Ke
%J Food and Agricultural Immunology
%D 2019
%R https://doi.org/10.1080/09540105.2019.1577365
%X ABSTRACT The flunixin residues in foods of animal origin could pose hazards for human health. In this paper, a broad-selectivity polyclonal antibody was obtained using 5-hydroxyflunixin coupled with bovine serum albumin as the immunogen. The semi-inhibitory concentration (IC50) of the polyclonal antibody was 1.43£¿ng/mL for flunixin and 0.29£¿ng/mL for 5-hydroxyflunixin, respectively, which were far below the maximum residue limits set by the United States, the European Union and China. The limits of detection were calculated to be 2.98£¿¦Ìg/kg for flunixin in bovine muscle and 0.78£¿¦Ìg/L for 5-hydroxyflunixin in milk. In spike and recovery tests, the average recovery ranged from 83% to 105%, with a satisfying coefficient of variance ranging from 5.8% to 11.3%. Furthermore, the ELISA method was validated by a well-established LC-MS/MS method. These results proved that the established method is suitable for flunixin and 5-hydroxyflunixin residue analysis
%U https://www.tandfonline.com/doi/full/10.1080/09540105.2019.1577365