%0 Journal Article
%T Bilberry anthocyanin-rich extract protects against retinal photooxidative damage via activation of HO-1 and inhibition of NF-¦ÊB
%A Aike Li
%A Hui Sun
%A Wentao Qi
%A Xiaoxuan Guo
%A Yong Wang
%J Food and Agricultural Immunology
%D 2019
%R https://doi.org/10.1080/09540105.2019.1637825
%X ABSTRACT Bilberry anthocyanins are functional ingredients for preventing retinal degeneration. This study was to determine whether the molecular mechanism of bilberry anthocyanin-rich extract (BAE) protection of retinal pigment epithelial (RPE) cells and retina against photooxidative damage via heme oxygenase-1 (HO-1) upregulation in vitro and in vivo. Results shown that BAE protected ARPE-19 cells from visible light-induced damage, reduced the intracellular reactive oxygen species levels, and up-regulated the expression of HO-1. Pigmented rabbits were treated with BAE (250 and 500£¿mg/kg/day) for 2 weeks pre-illumination and 1 week post-illumination until sacrifice. The expression of HO-1 in the retina was up-regulated after BAE treatment. BAE significantly suppressed the photooxidation-induced increase in expression of interleukine-6 protein. BAE also attenuated the expression of inflammation-related gene nuclear factor kappa B (NF-¦ÊB). Thus, BAE reduced retinal photooxidative damage through activating the HO-1 expression and suppressing NF-¦ÊB activation
%U https://www.tandfonline.com/doi/full/10.1080/09540105.2019.1637825