%0 Journal Article
%T L-Arginine protects ovine intestinal epithelial cells from lipopolysaccharide-induced intestinal barrier injury
%A Along Peng
%A Hao Zhang
%A Hongrong Wang
%A Juan J. Loor
%A Shuang Guo
%A Yaqian Jin
%J Food and Agricultural Immunology
%D 2019
%R https://doi.org/10.1080/09540105.2019.1664417
%X ABSTRACT This research aims to explore the effect of L-arginine (Arg) on ovine intestinal epithelial cells (IOECs) from lipopolysaccharide (LPS)-induced intestinal barrier injury. IOECs were incubated for 16£¿h and classified as 4 groups with the replacement of various mediums, including (1) control (Con), Arg-free Dulbecco's modified Eagle's F12 Ham medium (DMEM); (2) Arg treatment, Arg-free DMEM supplemented with 100£¿¦ÌM Arg; (3) LPS treatment, Arg-free DMEM supplemented with 10£¿¦Ìg/mL LPS; (4) LPS with Arg treatment, Arg-free DMEM supplemented with both 10£¿¦Ìg/mL LPS and 100£¿¦ÌM Arg. Arg supplementation to LPS-treated IOECs resulted in lower (P£¿<£¿0.05) relative Toll-like receptor 4 (TLR4) protein level. Arg supplementation to LPS-treated IOECs resulted in up-regulated (P£¿<£¿0.05) zonula occludens-1 (ZO-1), peptide transporter 1 (PEPT1) protein levels, and the ratio of phosphorylated mechanistic target of rapamycin (phospho-mTOR)-to-mTOR. The results show that Arg protects IOECs from LPS induced intestinal barrier injury
%U https://www.tandfonline.com/doi/full/10.1080/09540105.2019.1664417