%0 Journal Article
%T Development of an immunochromatography assay for salinomycin and methyl salinomycin in honey
%A Gang Cui
%A Hua Kuang
%A Liqiang Liu
%A Xiaoling Wu
%A Xiaoxin Xu
%J Food and Agricultural Immunology
%D 2019
%R https://doi.org/10.1080/09540105.2019.1649370
%X ABSTRACT This study developed a sensitive monoclonal antibody, 3A3, to salinomycin (SLM) and methyl salinomycin (MLN). The monoclonal antibody 3A3 is highly specific for SLM with an antibody halfinhibitory concentration (IC 50) of 0.86£¿ng/mL, a detection limit of 0.28£¿ng/mL, and a linear detection range of 0.28¨C2.26£¿ng/mL. The cross-reaction values of SLM and MLN were 100% and 74.48%, respectively. The recovery of honey samples ranged from 94.6% to 100.4%, thus indicating that this analytical method can be used for the analysis of real honey samples. In addition, immunochromatographic bands were developed for qualitative, semi-quantitative and speckle detection analysis. The critical values of SLM and MLN in honey were 40 and 40£¿ng/mL, respectively. The results showed that these analyses can be used for real samples, with the most stringent MRL of 40£¿ng/mL, for SLM and MLN
%U https://www.tandfonline.com/doi/full/10.1080/09540105.2019.1649370