%0 Journal Article
%T Development of a skeleton-specific antibody and Au nanoparticle-based immunochromatographic sensor for simultaneous detection of various tadalafil adulterants in health food
%A Fan He
%A Hong Wang
%A Hongtao Lei
%A Jinyi Yang
%A Lihua Deng
%A Tingting Zou
%A Xuecai Tan
%A Yuanming Sun
%A Yuanxin Tian
%A Yudong Shen
%A Zhenlin Xu
%J Food and Agricultural Immunology
%D 2019
%R https://doi.org/10.1080/09540105.2019.1585417
%X ABSTRACT In this work, a rapid, convenient and sensitive Au nanoparticle-based immunochromatographic sensor (AuNPs-ICS) for simultaneous detection of various tadalafil adulterants in health food was established for the first time, based on one high affinity and broad-spectrum antibody through skeleton-specific hapten design according to the common structure of tadalafil drugs. This immunochromatographic sensor was developed by the polyclonal antibody conjugated with AuNPs and the IC50 was 10.04£¿ng£¿mL£¿1 for tadalafil and 9.55, 11.46, 17.01£¿ng£¿mL£¿1 for amino tadalafil, acetamino tadalafil and nortadalafil, respectively. The limit of detection results could be obtained visually by naked eyes for and computationally by the hand-held immunochromatography reader. The sensor could specially detect Tadalafil and its analogs within 10£¿min in health food samples, and the close correlation with UPLC-MS/MS method showed the sensor was accurate and reliable, which could apply to on site detection Tadalafil and its analogs rapidly and accurately
%U https://www.tandfonline.com/doi/full/10.1080/09540105.2019.1585417