%0 Journal Article
%T Two strains of probiotic Lactobacillus enhance immune response and promote naive T cell polarization to Th1
%A Dayong Ren
%A Di Wang
%A Hansong Yu
%A Hongyan Liu
%A Minghao Shen
%J Food and Agricultural Immunology
%D 2019
%R https://doi.org/10.1080/09540105.2019.1579785
%X ABSTRACT We co-cultured Caco-2 cells with Lactobacillus salivarius and L. plantarum in vitro to investigate their immunoregulatory mechanisms and detected Lactobacillus strains adhering to Caco-2 cells expressing mRNAs of cytokines and Toll-like receptors (TLRs) by fluorescent quantitative reverse transcription¨Cpolymerase chain reaction. The strains were intragastrically administered to mice. Thymus/spleen indexes were measured on different days. 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay, flow cytometry, and enzyme-linked immunosorbent assay were performed to assess different variables. Results showed increased mRNA expressions of T helper 1 (Th1), various cytokines, surface receptors, TLRs (2, 4, and 9), thymus/spleen indexes, spleen lymphocyte transformation rate, macrophage energy metabolism and phagocytosis, CD11c+CD80+ cell number, interferon-¦Ã secretion, and sIgA secretion levels; inhibition of nuclear factor-¦ÊB inflammation signal pathway; and downregulated mRNA expression of specific interleukins. Both Lactobacillus strains can promote naive T cell differentiation (Th1) and participate in immunomodulatory responses, featuring a potential in prevention and management approaches for foodborne diseases
%U https://www.tandfonline.com/doi/full/10.1080/09540105.2019.1579785