%0 Journal Article
%T Dexmedetomidine inhibits the NF-¦ÊB pathway and NLRP3 inflammasome to attenuate papain-induced osteoarthritis in rats
%A Fang Cheng
%A Feng-Feng Yan
%A Ke-Fu Sun
%A Xue-Ming He
%A Yan Cong
%A Yue-Peng Liu
%J Pharmaceutical Biology
%D 2019
%R https://doi.org/10.1080/13880209.2019.1651874
%X Abstract Context: Dexmedetomidine (Dex) has been reported to have an anti-inflammatory effect. However, its role on osteoarthritis (OA) has not been explored. Objective: This study investigates the effect of Dex on OA rat model induced by papain. Materials and methods: The OA Wistar rat model was induced by intraluminal injection of 20£¿mL of papain mixed solution (4% papain 0.2£¿mL mixed with 0.03£¿mol L£¿1 l-cysteine 0.1£¿mL) into the right knee joint. Two weeks after papain injection, OA rats were treated by intra-articular injection of Dex (5, 10, or 20£¿¦Ìg kg£¿1) into the right knee (once a day, continuously for 4 weeks). Articular cartilage tissue was obtained after Dex treatment was completed. Results: The gait behavior scores (2.83£¿¡À£¿0.49), PWMT (15.2£¿¡À£¿1.78) and PTWL (14.81£¿¡À£¿0.92) in H-DEX group were higher than that of OA group, while Mankin score (5.5£¿¡À£¿0.81) was decreased (p£¿<£¿0.05). Compared with the OA group, the IL-1¦Â (153.11£¿¡À£¿16.05£¿pg mg£¿1), IL-18 (3.71£¿¡À£¿0.7£¿pg mg£¿1), IL-6 (14.15£¿¡À£¿1.94£¿pg/mg) and TNF-¦Á (40.45£¿¡À£¿10.28£¿pg mg£¿1) levels in H-DEX group were decreased (p£¿<£¿0.05). MMP-13, NLRP3, and caspase-1 p10 expression in Dex groups were significantly lower than that of OA group (p£¿<£¿0.05), while collagen II was increased (p£¿<£¿0.05). p65 in the nucleus of Dex groups was significantly down-regulated than that of OA group (p£¿<£¿0.05). Discussion and Conclusions: Dex can improve pain symptoms and cartilage tissue damage of OA rats, which may be related to its inhibition of the activation of NF-¦ÊB and NLRP3 inflammasome
%U https://www.tandfonline.com/doi/full/10.1080/13880209.2019.1651874