%0 Journal Article
%T Considerations towards a roadmap for collection, handling and storage of blood extracellular vesicles
%A Aled Clayton
%A Alice Gualerzi
%A An Hendrix
%A Andrew Hoffman
%A Carolina Soekmadji
%A Cecilia L£¿sser
%A Charlotte Lawson
%A Chris Gardiner
%A Dakota Gustafson
%A Edit I Buzas
%A Eric Boilard
%A Irina Nazarenko
%A Jennifer Jones
%A Joshua A Welsh
%A Juan Manual Falc¨®n-Perez
%A Ken Witwer
%A Lei Zheng
%A Lesley Cheng
%A Lorraine O¡¯Driscoll
%A Marca Wauben
%A Metka Lenassi
%A Pia R-M Silj
%A Rienk Nieuwl
%A Ryan Pink
%A er
%J Journal of Extracellular Vesicles
%D 2019
%R https://doi.org/10.1080/20013078.2019.1647027
%X ABSTRACT There is an increasing interest in exploring clinically relevant information that is present in body fluids, and extracellular vesicles (EVs) are intrinsic components of body fluids (¡°liquid biopsies¡±). In this report, we will focus on blood. Blood contains not only EVs but also cells, and non-EV particles including lipoproteins. Due to the high concentration of soluble proteins and lipoproteins, blood, plasma and serum have a high viscosity and density, which hampers the concentration, isolation and detection of EVs. Because most if not all studies on EVs are single-centre studies, their clinical relevance remains limited. Therefore, there is an urgent need to improve standardization and reproducibility of EV research. As a first step, the International Society on Extracellular Vesicles organized a biomarker workshop in Birmingham (UK) in November 2017, and during that workshop several working groups were created to focus on a particular body fluid. This report is the first output of the blood EV work group and is based on responses by work group members to a questionnaire in order to discover the contours of a roadmap. From the answers it is clear that most respondents are in favour of evidence-based research, education, quality control procedures, and physical models to improve our understanding and comparison of concentration, isolation and detection methods. Since blood is such a complex body fluid, we assume that the outcome of the survey may also be valuable for exploring body fluids other than blood
%U https://www.tandfonline.com/doi/full/10.1080/20013078.2019.1647027