%0 Journal Article
%T Impact of dietary methionine on respiratory tissue integrity and humoral immunity of broilers challenged with Mycoplasma gallisepticum and treated with Pulmotil AC£¿
%A Houssam Shaib
%A Mohamad T. Farran
%A Nour Ramadan
%A Zaynab Kaouk
%J Journal of Applied Animal Research
%D 2019
%R https://doi.org/10.1080/09712119.2019.1674659
%X ABSTRACT The experiment was performed to determine the effect of 20% dietary DL-Methionine (Met) in excess of modern breeder requirement level compared to Tilmicosin (Pulmotil AC£¿) on the humoral immune response and histopathological parameters of male broilers challenged with Mycoplasma gallisepticum (MG). Birds of Treatments 1¨C4 remained unchallenged while those of Treatments 5¨C8 were challenged with MG at 10 days of age. Diets with excess Met were formulated for birds of Treatments 3, 4, 7 and 8. Broilers of Treatments 2, 3, 6 and 7 were administered Pulmotil AC£¿ in drinking water. The 20% excess dietary Met significantly reduced tracheal deciliation, preserved the integrity of tracheal mucosal layer and significantly decreased goblet cell degeneration in MG-challenged birds. Furthermore, MG-challenged broilers administered 20% excess Met manifested higher ELISA titers against MG and a significantly higher antibody response to p33 (MG-cytadhesin putative gene) and p66 (MG-hemagglutinin protein A (pMGA)) in comparison to the other treatments. The 20% excess Met thereby maintained the health of the respiratory system and embodied an increased protection against MG-putative gene and a greater humoral response to MG challenge in broilers
%U https://www.tandfonline.com/doi/full/10.1080/09712119.2019.1674659