%0 Journal Article
%T Application of miR-193a/WT1/PODXL axis to estimate risk and prognosis of idiopathic membranous nephropathy
%A Jie Li
%A Wei Zhang
%A Yeping Ren
%J Renal Failure
%D 2019
%R https://doi.org/10.1080/0886022X.2019.1642210
%X Abstract Background: This investigation was managed to explore whether miR-193a in combination with two podocytes, namely, Wilms tumor type 1 (WT1) and podocalyxin (PODXL), were feasible in estimating onset and prognosis of idiopathic membranous nephropathy (IMN). Methods: We recruited a total of 189 healthy controls and 364 IMN patients, whose urine samples were prepared to measure the expression of miR-193a and PODXL. Meanwhile, renal tissues collected from above-mentioned IMN patients (n£¿=£¿364) and renal cell carcinoma patients (n£¿=£¿189) were arranged to determine the expression of WT1. Ultimately, receiver operating characteristic curves were constructed to appraise the performance of miR-193a, WT1, and PODXL in predicting renal survival of IMN patients. Results: The IMN patients were measured with up-regulated miR-193a expression and down-regulated WT1/PODXL expression, when compared with healthy controls (p£¿<£¿0.05). Moreover, highly expressed miR-193a, lowly expressed WT1/PODXL, elevated amounts of proteinuria (>3.79£¿g/24£¿h)/serum creatinine (>174.63£¿¦Ìmol/L), and declined GFR (¡Ü68.13£¿mL/min/1.73£¿m2) were implicated as prominent biomarkers for the poor renal survival of IMN patients (all p£¿<£¿0.05). Notably, miR-193a combined with PODXL and WT1 generated optimal effects in differentiating IMN patients from healthy controls (AUC£¿=£¿0.994) and also in anticipating the renal survival state of IMN patients (AUC£¿=£¿0.824), when compared with strategies that merely employed ¡Ü2 of the biomarkers. Conclusion: The combination of miR-193a, WT1, and PODXL might serve as a favorable strategy for expecting IMN prognosis
%U https://www.tandfonline.com/doi/full/10.1080/0886022X.2019.1642210