%0 Journal Article
%T High glucose and TGF-¦Â1 reduce expression of endoplasmic reticulum-resident selenoprotein S and selenoprotein N in human mesangial cells
%A Fumeng Huang
%A Lanmei Jing
%A Li Wang
%A Lifang Tian
%A Rongguo Fu
%A Shemin Lu
%A Yuanxu Guo
%A Zhao Chen
%J Renal Failure
%D 2019
%R https://doi.org/10.1080/0886022X.2019.1641413
%X Abstract There are seven endoplasmic reticulum (ER)-resident selenoproteins in human body and they can regulate the inflammation, oxidative stress, and ER stress. We established transforming growth factor-¦Â1 (TGF-¦Â1) or high glucose (HG) induced human mesangial cells (HMCs) fibronectin expression model in vitro. Next, the expression changes of seven ER-resident selenoproteins were detected under HG conditions and we found selenoprotein S (SELENOS), selenoprotein N (SELENON) were significantly down-regulated but selenoprotein M was significantly up-regulated in transcription level. Furthermore, we found that TGF-¦Â1 and HG down-regulated the expression of SELENOS and SELENON in a time- and dose-dependent manner, respectively. Finally, SELENOS was knocked down by siRNA and we found that knocking down SELENOS decreased TGF-¦Â1 induced fibronectin expression. Our research indicates the potential value of ER-resident selenoproteins on renal fibrosis
%U https://www.tandfonline.com/doi/full/10.1080/0886022X.2019.1641413