%0 Journal Article
%T The Yersinia pseudotuberculosis Cpx envelope stress system contributes to transcriptional activation of rovM
%A Dharmender K. Gahlot
%A Edvin J. Thanikkal
%A Ikenna R. Obi
%A Junfa Liu
%A Jyoti M. Gurung
%A Karl M. Thompson
%A Kristina Ruuth
%A Marcus Fredriksson Sundbom
%A Matthew S. Francis
%A Monika K. Francis
%A Petra Dersch
%A Shiyun Chen
%J Virulence
%D 2019
%R https://doi.org/10.1080/21505594.2018.1556151
%X ABSTRACT The Gram-negative enteropathogen Yersinia pseudotuberculosis possesses a number of regulatory systems that detect cell envelope damage caused by noxious extracytoplasmic stresses. The CpxA sensor kinase and CpxR response regulator two-component regulatory system is one such pathway. Active Cpx signalling upregulates various factors designed to repair and restore cell envelope integrity. Concomitantly, this pathway also down-regulates key determinants of virulence. In Yersinia, cpxA deletion accumulates high levels of phosphorylated CpxR (CpxR~P). Accumulated CpxR~P directly repressed rovA expression and this limited expression of virulence-associated processes. A second transcriptional regulator, RovM, also negatively regulates rovA expression in response to nutrient stress. Hence, this study aimed to determine if CpxR~P can influence rovA expression through control of RovM levels. We determined that the active CpxR~P isoform bound to the promoter of rovM and directly induced its expression, which naturally associated with a concurrent reduction in rovA expression. Site-directed mutagenesis of the CpxR~P binding sequence in the rovM promoter region desensitised rovM expression to CpxR~P. These data suggest that accumulated CpxR~P inversely manipulates the levels of two global transcriptional regulators, RovA and RovM, and this would be expected to have considerable influence on Yersinia pathophysiology and metabolism
%U https://www.tandfonline.com/doi/full/10.1080/21505594.2018.1556151