%0 Journal Article
%T Improved Raoultella planticola Strains for the Production of 2,3-Butanediol from Glycerol
%J Fermentation | An Open Access Journal from MDPI
%D 2019
%R https://doi.org/10.3390/fermentation5010011
%X Raw glycerol is an industrial byproduct from biodiesel production and is one of the most promising substrates for 2,3-butanediol (2,3-BD) production; however, 2,3-BD is not yet produced by fermentation from glycerol on a commercial scale due to poor process economics. Class 1 microorganism collections were screened and Raoultella planticola strain CECT 843 proved to be the best 2,3-BD producer, achieving (23.3 ¡À 1.4) g 2,3-BD per L and a yield of 36% (g 2,3-BD per g glycerol). To further increase product concentration and yield, R. planticola CEC T843 was subjected to random mutagenesis using ultra-violet (UV) light and ethyl methane sulfonate (EMS). Two mutant strains were found to produce at least 30% more 2,3-BD than the wild type: R. planticola IA1 [(30.8 ¡À 3.9) g 2,3-BD per L and 49% yield] and R. planticola IIIA3 [(30.5 ¡À 0.4) g 2,3-BD per L and 49% yield]. View Full-Tex
%U https://www.mdpi.com/2311-5637/5/1/11