%0 Journal Article
%T Viability of Neural Cells on 3D Printed Graphene Bioelectronics
%J Biosensors | An Open Access Journal from MDPI
%D 2019
%R https://doi.org/10.3390/bios9040112
%X Parkinson¡¯s disease (PD) is the second most common neurodegenerative disease in the United States after Alzheimer¡¯s disease (AD). To help understand the electrophysiology of these diseases, N27 neuronal cells have been used as an in vitro model. In this study, a flexible graphene-based biosensor design is presented. Biocompatible graphene was manufactured using a liquid-phase exfoliation method and bovine serum albumin (BSA) for further exfoliation. Raman spectroscopy results indicated that the graphene produced was indeed few-layer graphene (FLG) with ( I D / I G ) G r a p h e n e = 0.11. Inkjet printing of this few-layer graphene ink onto Kapton polyimide (PI) followed by characterization via scanning electron microscopy (SEM) showed an average width of ¡Ö868 ¦Ìm with a normal thickness of ¡Ö5.20 ¦Ìm. Neuronal cells were placed on a thermally annealed 3D printed graphene chip. A live¨Cdead cell assay was performed to prove the biosensor biocompatibility. A cell viability of approximately 80% was observed over 96 h, which indicates that annealed graphene on Kapton PI substrate could be used as a neuronal cell biosensor. This research will help us move forward with the study of N27 cell electrophysiology and electrical signaling. View Full-Tex
%U https://www.mdpi.com/2079-6374/9/4/112