%0 Journal Article
%T Biomass and Astaxanthin Productivities of Haematococcus pluvialis in an Angled Twin-Layer Porous Substrate Photobioreactor: Effect of Inoculum Density and Storage Time
%J Biology | An Open Access Journal from MDPI
%D 2019
%R https://doi.org/10.3390/biology8030068
%X The microalga Haematococcus pluvialis is mainly cultivated in suspended systems for astaxanthin production. Immobilized cultivation on a Twin-Layer porous substrate photobioreactor (TL-PSBR) has recently shown promise as an alternative approach. In Vietnam, a TL-PSBR was constructed as a low-angle (15 ∼) horizontal system to study the cultivation of H. pluvialis for astaxanthin production. In this study, the biomass and astaxanthin productivities and astaxanthin content in the dry biomass were determined using different initial biomass (inoculum) densities (from 2.5 to 10 g dry weight m ˋ2), different storage times of the initial biomass at 4 ∼C (24, 72, 120 and 168 h) and different light intensities (300每1000 米mol photons m ˋ2 s ˋ1). The optimal initial biomass density at light intensities between 400每600 米mol photons ˋ2 s ˋ1 was 5每7.5 g m ˋ2. Algae stored for 24 h after harvest from suspension for immobilization on the TL-PSBR yielded the highest biomass and astaxanthin productivities, 8.7 g m ˋ2 d ˋ1 and 170 mg m ˋ2 d ˋ1, respectively; longer storage periods decreased productivity. Biomass and astaxanthin productivities were largely independent of light intensity between 300每1000 米mol photons m ˋ2 s ˋ1 but the efficiency of light use per mole photons was highest between 300每500 米mol photons m ˋ2 s ˋ1. The astaxanthin content in the dry biomass varied between 2每3% (w/w). Efficient supply of CO 2 to the culture medium remains a task for future improvements of angled TL-PSBRs. View Full-Tex
%U https://www.mdpi.com/2079-7737/8/3/68