%0 Journal Article
%T Peptide¨CGold Nanoparticle Conjugates as Artificial Carbonic Anhydrase Mimics
%J Catalysts | An Open Access Journal from MDPI
%D 2019
%R https://doi.org/10.3390/catal9110903
%X We herein describe the design and synthesis of a catalytically active peptide¨Cgold nanoparticle conjugate (Pep-Au-NP) that binds Zn(II) within its peptide monolayer and develops carbonic anhydrase activity. Specifically, a modified variant of the ¦Â-sheet forming IHIHIQI-peptide (IHQ), which forms an interstrand 3-His Zn(II)-binding site, was used as a ligand for spherical gold nanoparticles (Au-NPs). The resulting immobilized peptide maintains its ability to form ¦Â-sheets, as determined by circular dichroism (CD)-spectroscopy and, thus, maintains its ability to form Zn(II)-binding sites. The addition of Zn(II)-ions to the peptide¨Cgold nanoparticle conjugates ( [email protected]) resulted in significant improvements in rates of ester hydrolysis of 4-nitrophenyl acetate (4-NPA) and the hydration of CO 2 compared to the unconjugated peptide variants. Recycling of the catalyst revealed that [email protected] remains intact with at least 94% of its initial activity after five rounds of CO 2 hydration. The herein reported results reveal that Pep-Au-NPs are able to perform reactions catalyzed by natural metalloenzymes and open up new possibilities for the implementation of these conjugates. View Full-Tex
%U https://www.mdpi.com/2073-4344/9/11/903