%0 Journal Article
%T Associations of Anti-Aquaporin 5 Autoantibodies with Serologic and Histopathological Features of Sj£¿gren's Syndrome
%A Hyun-Duck Kim
%A Jennifer Lee
%A Sumin Jeon
%A Sung-Hwan Park
%A Youngnim Choi
%J Journal of Clinical Medicine | An Open Access Journal from MDPI
%D 2019
%R https://doi.org/10.3390/jcm8111863
%X Biomarkers to stratify the complex and heterogeneous phenotypes of Sjogren¡¯s syndrome (SS) are needed. We aimed to validate the prevalence of anti-aquaporin 5 (AQP5) IgG in a non-Korean cohort and to optimize the method to screen the anti-AQP5 IgG. The study cohort included 111 primary SS and 43 non-SS Sj£¿gren's International Collaborative Clinical Alliance (SICCA) controls that were obtained from the Sj£¿gren's International Collaborative Clinical Alliance registry, in addition to 35 systemic lupus erythematosus (SLE) and 35 rheumatoid arthritis (RA) phenotypes. Anti-AQP5 IgG was screened by cell-based immunofluorescence cytochemistry (CB-IFC) assay in the absence or presence of epitope peptides, as well as by ELISA using the epitope peptides as coated antigens. Anti-AQP5 IgG specific to an E1 epitope was best at discriminating between SS and non-SS, and the two different methods (CB-IFC and ELISA) presented comparable performance in diagnostic accuracy (0.690 vs. 0.707). Notably, the SLE and RA groups had substantially lower levels of anti-AQP5 IgG than the SS group. In addition, the presence of anti-AQP5_E1 IgG was associated with serologic and histopathological features of SS. In conclusion, a similar prevalence of anti-AQP5 IgG was confirmed in a non-Korean cohort. Screening anti-AQP5 autoantibodies may help to form subgroups of SS for targeted therapy
%U https://www.mdpi.com/2077-0383/8/11/1863