%0 Journal Article
%T Antioxidant and Leishmanicidal Evaluation of Pulicaria Inuloides Root Extracts: A Bioguided Fractionation
%A Atteneri L¨®pez-Arencibia
%A Hamza Fadel
%A Ignacio A. Jim¨¦nez
%A Ines Sifaoui
%A Isabel L. Bazzocchi
%A Jacob Lorenzo-Morales
%A Jos¨¦ E. Pi£¿ero
%A Mar¨ªa Reyes-Batlle
%A Nabil Ghedadba
%A Samir Benayache
%J Pathogens | An Open Access Journal from MDPI
%D 2019
%R https://doi.org/10.3390/pathogens8040201
%X Leishmaniasis remains a major world health problem, and in particular, Algeria ranks second for the incidence of cutaneous leishmaniasis. Pulicaria inuloides is a well-known Arabian Peninsula medicinal plant. In the present study, the chloroform, ethyl acetate and n-butanol extracts from the roots of Pulicaria inuloides were analyzed for antioxidant activity and its correlation with the total phenolic and flavonoid contents. The highest antioxidant activity using a DPPH assay was showed by the ethyl acetate extract (IC 50 4.08 ¦Ìg/mL), which also had the highest total phenolic content (307.12 ¦ÌgAGE). Furthermore, P. inuloides root extracts were evaluated against Leishmania amazonensis and Leishmania donovani. The results highlighted the chloroform extract as the most active one against both tested Leishmania strains. A bioguided fractionation of the chloroform extract led to the isolation of (8 R:8 S)-(75:25 er)-10-isobutyryloxy-8,9-epoxy-thymol isobutyrate as the main bioactive component, showing a potent leishmanicidal activity on L. amazonensis promatigote and amastigote stages (IC 50 5.03 and 2.87 ¦ÌM, respectively) and a good selectivity index on murine macrophages (CC 50 19.37 ¦ÌM). This study provides the first report of the antioxidant and leishmanicidal activities of P. inuloides root extracts and the results point to this species as a source of potential bioactive agents. View Full-Tex
%U https://www.mdpi.com/2076-0817/8/4/201