%0 Journal Article
%T Association of TLR4 Polymorphisms, Expression, and Vitamin D with Helicobacter pylori Infection
%J Journal of Personalized Medicine | An Open Access Journal from MDPI
%D 2019
%R https://doi.org/10.3390/jpm9010002
%X Helicobacter pylori ( H. pylori) infection is the strongest recognized risk factor for gastric adenocarcinoma. Since previous observations have shown that polymorphisms in innate immune system genes, as well as vitamin D (VitD) levels, could modify the risk of infection with Helicobacter pylori ( H. pylori), we analyzed the relation between single nucleotide polymorphisms (SNPs) in TLRs ( TLR1, TLR2, TLR4) CD14, RUNX3 and VitD levels with H. pylori infection. A case-control study on four hundred sixty Lebanese individuals was conducted. Eleven SNPs in total were genotyped and gene expression analysis using real-time PCR was performed in white blood cells of a subsample of eight individuals. A total of 49% of the participants were affected. Although no direct association was found between the SNPs and H. pylori infection, rs4986790G>A and rs4986791T>C in TLR4 were negatively associated with VitD levels (¦Â = £¿0.371, p = 5 ¡Á 10 £¿3 and ¦Â = £¿0.4, p = 2 ¡Á 10 £¿3, respectively), which was negatively associated with H. pylori infection (OR = 0.01, p < 1 ¡Á 10 £¿3). TLR4 expression was 3¡Á lower in individuals with H. pylori compared with non-infected ( p = 0.01). TLR4 polymorphisms, expression, and VitD could be implicated in H. pylori infection and further development of gastric adenocarcinoma. View Full-Tex
%U https://www.mdpi.com/2075-4426/9/1/2