%0 Journal Article
%T A Simple, Reproducible, Inexpensive, Yet Old-Fashioned Method for Determining Phagocytic and Bactericidal Activities of Macrophages
%A Masakazu Kaneko
%A Masashi Emoto
%A Yoshiko Emoto
%J Archive of "Yonsei Medical Journal".
%D 2016
%R 10.3349/ymj.2016.57.2.283
%X Macrophages (M£¿) play a pivotal role in the protection system by recognizing and eliminating invading pathogenic bacteria. Phagocytosis and the killing of invading bacteria are major effector functions of M£¿. Although the phagocytic and bactericidal activities of M£¿ have been analyzed via several methods using a light microscope, a fluorescence microscope, or a fluorescence-activated cell sorter, expensive materials and equipment are usually required, and the methods are rather complicated. Moreover, it is impossible to determine both the phagocytic and bactericidal activities of M£¿ simultaneously using these methods. In this review, we describe a simple, reproducible, inexpensive, yet old-fashioned method (antibiotic protection assay) for determining the phagocytic and bactericidal activities of M£¿
%K Anti-bacterial agent
%K gentamicin
%K macrophage
%K phagocytosis
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4740517/