%0 Journal Article
%T Effect of Citrus macroptera Fruit Pulp Juice on Alteration of Caspase Pathway Rendering Anti-Proliferative Activity against Ehrlich¡¯s Ascites Carcinoma in Mice
%A Ariful Haque
%A Kazi Md. Faisal Hoque
%A Md Abu Reza
%A Md. Mahmudul Hasan
%A Md. Shihabul Islam
%J Archive of "Toxicological Research".
%D 2019
%R 10.5487/TR.2019.35.3.271
%X Citrus macroptera (Rutaceae) has long been used in folk medicine in Bangladesh. Considering the folkloric context, this study was aimed to scrutinize anti-proliferative activity of C. macroptera fruit pulp juice (CMFPJ) against Ehrlich¡¯s ascites carcinoma (EAC). The anti-proliferative capacity of CMFPJ was investigated and confirmed primarily using MTT assay. In vivo anti-proliferative aptitude of CMFPJ was investigated with 25, 50, and 100 mg/kg/day intraperitoneal (i.p.) treatment. Anti-proliferative efficacy of CMFPJ was assessed based on EAC growth inhibition. CMFPJ inhibited EAC growth in vitro in a dose-dependent manner. And the percentages of in vivo EAC growth inhibition were 19.53, 49.2, and 68.9% at 25, 50, and 100 mg/kg CMFPJ respectively. CMFPJ significantly induced expression of apoptosis regulatory genes caspase-8, caspase-9, cytochrome-c, and caspase-3. This considerable anti-cancer activity was perhaps due to combinatorial effect of lectin, polyphenols, and flavonoids present in CMFPJ
%K Citrus macroptera
%K Extrinsic pathway
%K Intrinsic pathway
%K Apoptosis
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629448/