%0 Journal Article
%T Cytotoxicity Evaluation of Essential Oil and its Component from Zingiber officinale Roscoe
%A Yongkyu Lee
%J Archive of "Toxicological Research".
%D 2016
%R 10.5487/TR.2016.32.3.225
%X Zingiber officinale Roscoe has been widely used as a folk medicine to treat various diseases, including cancer. This study aims to re-examine the therapeutic potential of co-administration of natural products and cancer chemotherapeutics. Candidate material for this project, ¦Á-zingiberene, was extracted from Zingiber officinale Roscoe, and ¦Á-zingiberene makes up 35.02 ¡À 0.30% of its total essential oil. ¦Á-Zingiberene showed low IC50 values, 60.6 ¡À 3.6, 46.2 ¡À 0.6, 172.0 ¡À 6.6, 80.3 ¡À 6.6 (¦Ìg/mL) in HeLa, SiHa, MCF-7 and HL-60 cells each. These values are a little bit higher than IC50 values of general essential oil in those cells. The treatment of ¦Á-zingiberene produced nucleosomal DNA fragmentation in SiHa cells, and the percentage of sub-diploid cells increased in a concentration-dependent manner in SiHa cells, hallmark features of apoptosis. Mitochondrial cytochrome c activation and an in vitro caspase-3 activity assay demonstrated that the activation of caspases accompanies the apoptotic effect of ¦Á-zingiberene, which mediates cell death. These results suggest that the apoptotic effect of ¦Á-zingiberene on SiHa cells may converge caspase-3 activation through the release of mitochondrial cytochrome c into cytoplasm. It is considered that anti-proliferative effect of ¦Á-zingiberene is a result of apoptotic effects, and ¦Á-zingiberene is worth furthermore study to develop it as cancer chemotherapeutics
%K Cytotoxic activity
%K ¦Á-Zingiberene
%K Anti-proliferation
%K General essential oil
%K Cervix cancer cells
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4946420/