%0 Journal Article
%T Attenuated Phenotype and Immunogenic Characteristics of a Mutated Herpes Simplex Virus 1 Strain in the Rhesus Macaque
%A Fengmei Yang
%A Jianbin Wang
%A Lichun Wang
%A Min Feng
%A Nigel W. Fraser
%A Qihan Li
%A Shengtao Fan
%A Xingli Xu
%A Ying Zhang
%A Yongrong Wang
%A Yun Liao
%A Zhanlong He
%J Archive of "Viruses".
%D 2018
%R 10.3390/v10050234
%X Herpes simplex virus type 1(HSV-1) presents a conundrum to public health worldwide because of its specific pathogenicity and clinical features. Some experimental vaccines, such as the recombinant viral glycoproteins, exhibit the viral immunogenicity of a host-specific immune response, but none of these has achieved a valid epidemiological protective efficacy in the human population. In the present study, we constructed an attenuated HSV-1 strain M3 through the partial deletion of UL7, UL41, and the latency-associated transcript (LAT) using the CRISPR/Cas9 system. The mutant strain exhibited lowered infectivity and virulence in macaques. Neutralization testing and ELISpot detection of the specific T-cell responses confirmed the specific immunity induced by M3 immunization and this immunity defended against the challenges of the wild-type strain and restricted the entry of the wild-type strain into the trigeminal ganglion. These results in rhesus macaques demonstrated the potential of the attenuated vaccine for the prevention of HSV-1 in humans
%K HSV-1
%K mutant strain
%K immunogenicity
%K rhesus macaques
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977227/