%0 Journal Article
%T Sex-specific deficits in neurite density and white matter integrity are associated with targeted disruption of exon 2 of the Disc1 gene in the rat
%A Brian R. Barnett
%A C. Dustin Rubinstein
%A Emily A. Sawin
%A Jacqueline M. Anderson
%A John-Paul J. Yu
%A Kathleen Krentz
%A Maribel Torres-Vel¨¢zquez
%A Paul A. Rowley
%A Sue Y. Yi
%A Vaishali P. Bakshi
%J Archive of "Translational Psychiatry".
%D 2019
%R 10.1038/s41398-019-0429-2
%X a The selected strategy was a redundant 2-target CRPSPR/Cas9 approach (low transcript complexity, large targetable N-terminus exon). Two target sites in exon 2 were selected in the second coding exon. b Expected target 1 and 2 cleavage sites are indicated (arrowhead), and target 1 and 2 PAMs (green) are on the sense and antisense strand, respectively. Line #860 carried a 371£¿bp excision, verified by Sanger sequencing. c PCR of the target region generated a wild-type band for the unedited Sprague-Dawley (SD) sample, two bands for the #860 sample corresponding to the wild-type band and the expected deletion, and no bands for the no-template control (NTC). d Western blot of Disc1 protein from Sprague¨CDawley and Disc1 sv¦¤2 rat brain samples, performed with DSMS/D22 rabbit anti-DISC1 598-785 antibody. From left to right: Sprague-Dawley female rat, Disc1 sv¦¤2 female rat, Sprague-Dawley male rat, and Disc1 sv¦¤2 male rat. Western blotting shows absence of an endogenous Disc1 sv¦¤2 isoform in male and female animals (red arrow) in Disc1 sv¦¤2 male and female sample
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370885/