%0 Journal Article
%T Proteogenomic Identification of a Novel Protein-Encoding Gene in Bovine Herpesvirus 1 That Is Expressed during Productive Infection
%A Bindu Nanduri
%A Clinton Jones
%A Florencia Meyer
%A Fouad S. El-mayet
%A Kaley A. Barber
%A Victoria A. Jefferson
%J Archive of "Viruses".
%D 2018
%R 10.3390/v10090499
%X Bovine herpesvirus 1 (BoHV-1) is one of several microbes that contributes to the development of the bovine respiratory disease (BRD) and can also induce abortions in cattle. As other alpha-herpesvirinae subfamily members, BoHV-1 efficiently replicates in many cell types and subsequently establishes a life-long latent infection in sensory neurons. BoHV-1 encodes more than 70 proteins that are expressed in a well-defined manner during productive infection. However, in silico open reading frame (ORF) prediction of the BoHV-1 genome suggests that the virus may encode more than one hundred proteins. In this study we used mass spectrometry followed by proteogenomic mapping to reveal the existence of 92 peptides that map to previously un-annotated regions of the viral genome. Twenty-one of the newly termed ˇ°intergenic peptidesˇ± were predicted to have a viable ORF around them. Twelve of these produced an mRNA transcript as demonstrated by strand-specific RT-PCR. We further characterized the 5ˇä and 3ˇä termini of one mRNA transcript, ORF-A, and detected a 55 kDa protein produced during active infection using a custom-synthesized antibody. We conclude that the coding potential of BoHV-1 is underestimated
%K Bovine herpesvirus
%K proteogenomic mapping
%K gene discovery
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164122/