%0 Journal Article
%T Skin Corrosion and Irritation Test of Nanoparticles Using Reconstructed Three-Dimensional Human Skin Model, EpiDermTM
%A Byung-Il Yoon
%A Handule Lee
%A Hyejin Kim
%A Jonghye Choi
%A Juyoung Park
%A Kwangsik Park
%A Seon Mi Jin
%J Archive of "Toxicological Research".
%D 2016
%R 10.5487/TR.2016.32.4.311
%X Effects of nanoparticles (NPs) on skin corrosion and irritation using three-dimensional human skin models were investigated based on the test guidelines of Organization for Economic Co-operation and Development (OECD TG431 and TG439). EpiDermTM skin was incubated with NPs including those harboring iron (FeNPs), aluminum oxide (AlNPs), titanium oxide (TNPs), and silver (AgNPs) for a defined time according to the test guidelines. Cell viabilities of EpiDermTM skins were measured by the 3-(4, 5-dimethylthi-azol-2-yl)-2.5-diphenyltetrazolium bromide based method. FeNPs, AlNPs, TNPs, and AgNPs were non-corrosive because the viability was more than 50% after 3 min exposure and more than 15% after 60 min exposure, which are the non-corrosive criteria. All NPs were also non-irritants, based on viability exceeding 50% after 60 min exposure and 42 hr post-incubation. Release of interleukin 1-alpha and histopathological analysis supported the cell viability results. These findings suggest that FeNPs, AlNPs, TNPs, and AgNPs are ¡®non-corrosive¡¯ and ¡®non-irritant¡¯ to human skin by a globally harmonized classification system
%K Nanoparticles
%K Skin model
%K Alternative methods
%K Skin corrosion
%K Skin irritation
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5080854/