%0 Journal Article
%T Intrabeam Radiation Inhibits Proliferation, Migration, and Invasiveness and
Promotes Apoptosis of MCF-7 Breast Cancer Cells
%A Changsheng Ye
%A Lingxiao Pan
%A Minghui Wan
%A Rui Wu
%A Tong Yang
%A Wei Tang
%A Wenbo Zheng
%A Xiaoshen Zhang
%J Archive of "Technology in Cancer Research & Treatment".
%D 2019
%R 10.1177/1533033819840706
%X Intraoperative radiotherapy differs from the more commonly used external beam radiation with respect to fractionation, radiation energy, dose rate, and target volume, which may influence the irradiated cells in a complex manner. However, experimental studies of intraoperative radiotherapy are limited. Intrabeam is a frequently used intraoperative radiotherapy device; we evaluated its effects on the proliferation, apoptosis, migration, and invasion of MCF-7 human breast cancer cells. We performed colony formation assays for cells irradiated with single radiation doses of 0 to 16 Gy. Other cells were irradiated with single radiation doses of 0 to 6 Gy and then continued to be cultured. We measured cell-cycle distributions and apoptosis rates 24 hours later, using flow cytometry, and performed wound-healing assays, Transwell tests, and terminal deoxynucleotidyl transferase¨Cmediated 2¡ä-deoxyuridine 5¡ä-triphosphate nick-end labeling staining 4 weeks later. Colony formation assays showed no positive colonies from cells irradiated with doses of ¡Ý6 Gy. In flow cytometry, the experimental groups had higher late-apoptosis/necrosis rates (P < .01) and higher percentages of cells arrested in G1 phase (P < .01). Experimental groups also had much lower scratch-repair rates in the wound healing assay (P < .001) and higher apoptosis rates in the terminal deoxynucleotidyl transferase¨Cmediated 2¡ä-deoxyuridine 5¡ä-triphosphate nick-end labeling assay (P < .05). In Transwell tests, the 4 Gy and 6 Gy groups had fewer invading cells than the control group (P < .05). Single-dose irradiation of 6 Gy with the Intrabeam device can effectively inhibit proliferation, migration, and invasiveness and promote apoptosis in MCF-7 cells with long-lasting effects
%K breast cancer
%K irradiation
%K proliferation
%K migration
%K invasion
%K apoptosis
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444775/