%0 Journal Article %T Design of a comprehensive microfluidic and microscopic toolbox for the ultra-wide spatio-temporal study of plant protoplasts development and physiology %A Fabien Nogu¨¦ %A Florence Charlot %A Jacques Fattaccioli %A Jean-Christophe Palauqui %A Kaori Sakai %A Sandrine Bonhomme %A Thomas Le Saux %J Archive of "Plant Methods". %D 2019 %R 10.1186/s13007-019-0459-z %X The online version of this article (10.1186/s13007-019-0459-z) contains supplementary material, which is available to authorized users %K Microfluidics %K Physcomitrella patens %K Regeneration %K Development %K Differentiation %K Protoplasts %K Spores %K Microscopy %U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651895/