%0 Journal Article
%T Optimizing Suitable Antibiotics for Bacterium Control in Micropropagation of Cherry Rootstock Using a Modified Leaf Disk Diffusion Method and E Test
%A Chenglin Liang
%A Rendun Wu
%A Tian Wan
%A Yu Han
%A Yuliang Cai
%J Archive of "Plants".
%D 2019
%R 10.3390/plants8030066
%X Bacterial contamination is a major and constant threat to the establishment and subculture of in vitro plant culture. In this study, we used a slightly modified qualitative disk diffusion method to screen optimal antibiotics to control the growth of bacterial contaminants isolated from explants of cherry rootstock ¡®Gisela 6¡¯. Bacterial susceptibility to eight different antibiotics was tested. The results showed that tetracycline was the most effective antibiotic for controlling bacterial growth; cefotaxime, carbenicillin, kanamycin, and streptomycin were less effective, whereas ampicillin, penicillin, and cefazolin did not inhibit growth of the isolated bacteria. Using the quantitative E test, the minimal inhibitory concentration (MIC) of tetracycline was determined to be 1.0 ¦Ìg mL£¿1. We also measured the Fv/Fm values, chlorophyll content, and enzymatic activity of superoxide dismutase and peroxidase to explore the effect of different tetracycline concentrations, 0, 0.064, 0.5, 1.0, 16, and 256 ¦Ìg mL£¿1, on the growth of bacteria and explants over 30 days. Results indicated that 1.0 ¦Ìg mL£¿1 tetracycline was effective in restricting bacterial growth, with non-significant negative effects on explants at low concentrations, but were enhanced negative effects at high concentrations. The application of the disk diffusion method and E test enabled the identification of an antibiotic and its MIC value effective for eliminating bacterial contaminants while causing minimal damage to explants, indicating a high potential of these methods to control bacterial contaminants in in vitro plant culture
%K antibiotics
%K bacterial contaminants
%K disk diffusion
%K E test
%K in vitro plant culture
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6473490/