%0 Journal Article
%T Real-time monitoring of PtaHMGB activity in poplar transactivation assays
%A Alicia Moreno-Cort¨¦s
%A Daniel Conde
%A Isabel Allona
%A Jos¨¦ M. Ramos-S¨˘nchez
%A Mariano Perales
%A Paolo M. Triozzi
%J Archive of "Plant Methods".
%D 2017
%R 10.1186/s13007-017-0199-x
%X Precise control of gene expression is essential to synchronize plant development with the environment. In perennial plants, transcriptional regulation remains poorly understood, mainly due to the long time required to perform functional studies. Transcriptional reporters based on luciferase have been useful to study circadian and diurnal regulation of gene expression, both by transcription factors and chromatin remodelers. The high mobility group proteins are considered transcriptional chaperones that also modify the chromatin architecture. They have been found in several species, presenting in some cases a circadian expression of their mRNA or protein
%K Transcriptional reporter
%K Transactivation assay
%K Chromatin remodeling
%K High mobility group
%K Diurnal and circadian rhythms
%K Poplar
%K Populus tremula ˇÁ alba
%K Transient expression
%K LHY2
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5472981/