%0 Journal Article
%T TALEN-mediated genome-editing approaches in the liverwort Marchantia polymorpha yield high efficiencies for targeted mutagenesis
%A Esther Sch¨¹£¿ler
%A Felix Althoff
%A Sabine Zachgo
%A Sarah Kopischke
%J Archive of "Plant Methods".
%D 2017
%R 10.1186/s13007-017-0167-5
%X The liverwort Marchantia polymorpha occupies a crucial position in land plant evolution and provides the opportunity to investigate adaptations to a terrestrial plant life style. Marchantia reverse genetic analyses have thus far been conducted by employing a homologous recombination approach, which yields an efficiency of around 3%. Availability of the characterized and suitable endogenous MpEF1¦Á promoter prompted us to establish the TALEN gene targeting technique for Marchantia
%K TALEN
%K Genome editing
%K Marchantia polymorpha
%K MpNOP1
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5370431/