%0 Journal Article
%T The combination of gas-phase fluorophore technology and automation to enable high-throughput analysis of plant respiration
%A A. Clarissa A. Negrini
%A A. Harvey Millar
%A Andrew P. Scafaro
%A Brendan OˇŻLeary
%A F. Azzahra Ahmad Rashid
%A Lucy Hayes
%A Murray R. Badger
%A Owen K. Atkin
%A Vincent Chochois
%A You Zhang
%A Yuzhen Fan
%J Archive of "Plant Methods".
%D 2017
%R 10.1186/s13007-017-0169-3
%X Mitochondrial respiration in the dark (R dark) is a critical plant physiological process, and hence a reliable, efficient and high-throughput method of measuring variation in rates of R dark is essential for agronomic and ecological studies. However, currently methods used to measure R dark in plant tissues are typically low throughput. We assessed a high-throughput automated fluorophore system of detecting multiple O2 consumption rates. The fluorophore technique was compared with O2-electrodes, infrared gas analysers (IRGA), and membrane inlet mass spectrometry, to determine accuracy and speed of detecting respiratory fluxes
%K Dark respiration
%K Fluorophore
%K Gas-exchange
%K High-throughput
%K Oxygen consumption
%K Oxygen electrodes
%K Respiration
%K Respiratory flux
%K Respiratory quotient
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5361846/