%0 Journal Article
%T Characterization of protein arginine methyltransferase of TgPRMT5 in Toxoplasma gondii
%A Chun-Yuan Li
%A Fen-Xiang Li
%A Kun Wu
%A Liwang Cui
%A Long-Fei Chen
%A Min Liu
%A Pei-Liang Yang
%A Ting-kai Liu
%A William J. Sullivan
%A Jr.
%A Xiao-Cong Li
%A Xiao-Guang Chen
%A Zhi-Fa Lai
%J Archive of "Parasites & Vectors".
%D 2019
%R 10.1186/s13071-019-3464-1
%X Confirmation of the C-terminal HA-tagging of the endogenous TgPRMT5 locus in the Pru strain. a Predicted integration event of HA fusion at the endogenous TgPRMT5 locus. Top: TgPRMT5 locus on chromosome X. Grey boxes represent the front part of TgPRMT5 gene; white boxes represent the TgPRMT5 region used for homologous recombination in the transfection plasmid; red boxes indicate HAx3-tagged. Middle: the plasmid pLIC_PRMT5_HAx3_DHFRTs. Bottom: The resultant single-crossover event at the TgPRMT5 locus with integration of one copy of the plasmid. b Western blot with anti-HA antibody that bind to the HA-tag. The band of 110 kDa was detected in the HA-tagged TgPRMT5 clone. Lane 1: clone of HA-tagged TgPRMT5; Lane 2: wild-type Pru strain as negative control. c Expression of TgPRMT5 in tachyzoites and bradyzoites. The two stages of the parasite were differentiated by antibody against BAG1, a protein expressed specifically in the bradyzoite stage. Anti-¦Â-tubulin antibody served as a protein loading contro
%K Histone
%K Chromatin
%K Parasites
%K Epigenetics
%K Methylation
%K Bradyzoites
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6505072/