%0 Journal Article
%T Daikenchuto (TUİ\100) alters murine hepatic and intestinal drug metabolizing enzymes in an in£żvivo dietary model: effects of gender and withdrawal
%A Atsushi Kaneko
%A Eugene B. Chang
%A Hyunyoung Jeong
%A Jun Miyoshi
%A Junko Watanabe
%A Kentaro Nobutani
%A Mark W. Musch
%A Masahiro Yamamoto
%A Masaru Yoshida
%A Mitsue Nishiyama
%A Toru Kono
%J Archive of "Pharmacology Research & Perspectives".
%D 2017
%R 10.1002/prp2.361
%X Herbal medicines and natural products used for maintenance of health or treatment of diseases have many biological effects, including altering the pharmacokinetics and metabolism of other medications. Daikenchuto (TUİ\100), an aqueous extract of ginger, ginseng, and Japanese green pepper fruit, is a commonly prescribed Kampo (Japanese herbal medicine) for postoperative ileus or bloating. The effects of TUİ\100 on drug metabolism have not been investigated. In this study, we analyzed the effect of TUİ\100 on expression of key drugİ\metabolizing enzymes (DMEs) and drug transporters (DTs) in murine liver and gastrointestinal tract using a dietary model. Liver, jejunum, and proximal colon were analyzed for phase I and II DMEs and DT mRNA expression by reverse transcription (RT) first by nonquantitative and followed by quantitative polymerase chain reaction (PCR) and protein expression. Liver, jejunum, and proximal colon expressed some identical but also unique DMEs and DTs. TUİ\100 increased the greatest changes in cytochrome (Cyp) 2b10 and Cyp3a11 and Mdr1a. Basal and TUİ\100 stimulated levels of DME and DT expression were genderİ\dependent, doseİ\dependent and reversible after cessation of TUİ\100 supplementation, except for some changes in the intestine. Quantitative Western blot analysis of protein extracts confirmed the quantitative PCR results
%K Drug metabolizing enzymes
%K drug transporters
%K ginger
%K ginseng
%K sansho pepper
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5625165/