%0 Journal Article
%T Gestation changes sodium pump isoform expression, leading to changes in ouabain sensitivity, contractility, and intracellular calcium in rat uterus
%A Ali Mobasheri
%A Rachel V. Floyd
%A Susan Wray
%J Archive of "Physiological Reports".
%D 2017
%R 10.14814/phy2.13527
%X Developmental and tissue坼specific differences in isoforms allow Na+, K+坼ATPase function to be tightly regulated, as they control sensitivity to ions and inhibitors. Uterine contraction relies on the activity of the Na+, K+ ATPase, which creates ionic gradients that drive excitation坼contraction coupling. It is unknown whether Na+, K+ ATPase isoforms are regulated throughout pregnancy or whether they have a direct role in modulating uterine contractility. We hypothesized that gestation坼dependent differential expression of isoforms would affect contractile responses to Na+, K+ ATPase 汐 subunit inhibition with ouabain. Our aims were therefore: (1) to determine the gestation坼dependent expression of mRNA transcripts, protein abundance and tissue distribution of Na+, K+ ATPase isoforms in myometrium; (2) to investigate the functional effects of differential isoform expression via ouabain sensitivity; and (3) if changes in contractile responses can be explained by changes in intracellular [Ca2+]. Changes in abundance and distribution of the Na+, K+ ATPase 汐, 汕 and FXYD1 and 2 isoforms, were studied in rat uterus from nonpregnant, and early, mid坼, and term gestation. All 汐, 汕 subunit isoforms (1,2,3) and FXYD1 were detected but FXYD2 was absent. The 汐1 and 汕1 isoforms were unchanged throughout pregnancy, whereas 汐2 and 汐3 significant decreased at term while 汕2 and FXYD1 significantly increased from mid坼term onwards. These changes in expression correlated with increased functional sensitivity to ouabain, and parallel changes in intracellular Ca2+, measured with Indo坼1. In conclusion, gestation induces specific regulatory changes in expression of Na+, K+ ATPase isoforms in the uterus which influence contractility and may be related to the physiological requirements for successful pregnancy and delivery
%K Gestation
%K immunohistochemistry
%K isoform
%K K+坼ATPase
%K myometrium
%K Na+
%K smooth muscle
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727280/