%0 Journal Article
%T In vitro regeneration and Agrobacterium-mediated transformation of male- sterile marigold (Tagetes erecta L.)
%A Mana Narushima
%A Mikiko Uesugi
%A Nuoendagula
%A Shinya Kajita
%A Yasushi Murai
%A Yoshihiro Katayama
%A Yosuke Iimura
%J Archive of "Plant Biotechnology".
%D 2017
%R 10.5511/plantbiotechnology.17.0530a
%X In this study, we devised a method for the in vitro regeneration and subsequent genetic transformation of male sterile marigold. To our knowledge, this is the first report of generation of transgenic plants with a single genotype of marigold via Agrobacterium-mediated transformation. We obtained four transgenic lines from two independent experiments with 496 leaf explants, which were inoculated by an Agrobacterium strain LBA4404 harboring the plasmid, pIG121-Hm. Although the efficiency of the transformation in our system was low, stable expression of uidA gene in adventitious shoots and compound leaves could be detected in ¦Â-glucuronidase histochemical analysis. This protocol contributes to the progress of genetic studies and molecular breeding of this species
%K Agrobacterium
%K genetic transformation
%K male sterility
%K meropenem
%K Tagetes erecta
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6543758/