%0 Journal Article
%T Endothelial cell activation on 3D-matrices derived from PDGF-BB-stimulated fibroblasts is mediated by Snail1
%A Alberto Herrera
%A Alberto Mu£¿oz
%A Alfredo Carrato
%A Antonio Candia
%A Antonio Garc¨ªa de Herreros
%A Beatriz Gil
%A Cristina Galindo-Pumari£¿o
%A Cristina Pe£¿a
%A F¨¦lix Bonilla
%A Jes¨²s Pinto
%A Mar¨ªa Jes¨²s Larriba
%A Mercedes Herrera
%A Ma Laura Garc¨ªa-Bermejo
%A Natalia Guerra-Perez
%A Pilar Veguillas
%A Ra¨²l Pe£¿a
%A Reyes Ferreiro-Monteagudo
%A Sara Gim¨¦nez-Moyano
%A Vanesa Garc¨ªa-Barber¨¢n
%J Archive of "Oncogenesis".
%D 2018
%R 10.1038/s41389-018-0085-z
%X a Binary images representative of the cellular orientation of the BJ-hTERT fibroblasts treated or not with PDGF-BB (above) and directionality histograms (below) calculated with the Image J software which represent the frequency of distribution of cell angles (centered on the 0 ¡ã angle). b Increase in protein expression of extracellular proteins such as fibronectin and Collagen I in PDGF-stimulated fibroblasts derived from ECM. c Increase of ECM thickness, determined by confocal microscopy, in those ECMs derived from PDGF-stimulated fibroblasts. d Increase in protein and organization of extracellular proteins such as fibronectin and Collagen I in PDGF-stimulated fibroblasts derived from ECM. Under the image its represented the directionality histograms calculated with the Image J software which represent the frequency of distribution of protein fibers angles (centered on the 0 ¡ã angle). e, f Endothelial cell activation was observed by formation of capillary-like structures on matrices derived from PDGF-stimulated fibroblasts (e) and increased expression of angiogenesis-related markers (f). g Increase expression of ¦Â1 and ¦Â3 integrins subunits in HUVECs from PDGF-derived matrices. *p value£¿<£¿0,05; ***p value£¿<£¿0,001. All results are derived from 2¨C4 independent experiments, each performed in duplicat
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155204/