%0 Journal Article
%T BRAF inhibition causes resilience of melanoma cell lines by inducing the secretion of FGF1
%A Andreas Borst
%A Anita Hufnagel
%A Johannes Grimm
%A Marion Wobser
%A Roland Houben
%A Sebastian Haferkamp
%A Svenja Meierjohann
%J Archive of "Oncogenesis".
%D 2018
%R 10.1038/s41389-018-0082-2
%X a Conditioned supernatant (cond. SN) was generated from melanoma cells treated for 3 days with vemurafenib (vem, 0.5£¿¦ÌM) and from DMSO-treated control cells (ctrl). Donor cells were seeded to achieve an equal confluency at day 3. After excessive washing with PBS, donor cells were starved over night with medium containing 2% dialysed FCS. The following day, acceptor cells (which were starved for 3 days) were treated with filtered conditioned supernatant for 48£¿h followed by MTT measurement. b Western blot showing P-ERK1/2 (Thr202/Tyr204) of UACC-62 cells treated for 3 days with indicated concentrations of vemurafenib. Actin served as loading control. c MTT assays of the melanoma cell lines M14, UACC62, A375 and the fibroblast cell line WI-38 treated for 48£¿h with DMSO (¡°ctrl SN¡±) and vemurafenib-conditioned supernatant (¡°vem SN¡±). mel: melanoma; fb: fibroblast. Data are derived from three independent experiments. *p£¿<£¿0.05; **p£¿<£¿0.01; ***p£¿<£¿0.001 (Student¡¯s t-test, unpaired, comparison between vemurafenib-conditioned and control conditioned supernatant, which was set as 100%
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147791/