%0 Journal Article
%T FGF1 induces resistance to chemotherapy in ovarian granulosa tumor cells through regulation of p53 mitochondrial localization
%A Arnaud Guillaume
%A Bernard Mignotte
%A Caroline Pirou
%A Flore Renaud
%A Nathalie Le Floch
%A Sevasti Manousakidi
%A Sylvina Bouleau
%J Archive of "Oncogenesis".
%D 2018
%R 10.1038/s41389-018-0033-y
%X a Upper panel: Average flow cytometry quantification of apoptotic cells characterized by their low DIOC staining and cell condensation (DIOC-, Size-)£¿¡À£¿SEM for 3 experiments done in triplicate. Non-transfected COV434 (NT), two COV434-Mock clonal cell lines and three COV434-FGF1 clonal cell lines were treated with etoposide (25£¿¦Ìg/mL) for 16£¿h, or not treated (Ctl), The t-tests compare to NT Eto. Lower panel: FGF1 levels in non-transfected, mock and FGF1 overexpressing clones using western blot analysis. Total proteins are visualized with the Biorad stain free system. b Immunofluorescence study for cytochrome c release. COV434-Mock C1 and -FGF1 C1 cells were treated with 25£¿¦Ìg/mL etoposide for 4£¿h. Cells were stained with an anti-cytochrome c antibody (green) and TO-PRO-3 (blue) to visualize nuclei (left panels). Scale bar represents 40£¿¦Ìm. The histogram presents the average percentages£¿¡À£¿SEM for 3 independent experiments of cells exhibiting cytochrome c release (right panel). The t-test compares to Mock cells similarly treated. c Upper panel: Western blot analysis of total proteins for procaspase-9, cleaved caspase-9 and £¿3 and PARP levels. COV434-Mock and -FGF1 cells were treated or not (0£¿h) with 25£¿¦Ìg/mL etoposide for 2, 4, 6, or 16£¿h. Lower panel: histograms present the average fold-change decrease of cleaved caspase-9, cleaved caspase-3 and cleaved PARP in COV434-FGF1 cells£¿¡À£¿SEM from pooled results of three FGF1 overexpressing clones (n£¿=£¿8). Two-tailed unpaired t-tests results are shown as * for P£¿¡Ü£¿0.05, ** for P£¿¡Ü£¿0.01 and *** for P£¿¡Ü£¿0.00
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5833868/