%0 Journal Article
%T T©\cell redeployment and intracellular cytokine expression following exercise: effects of exercise intensity and cytomegalovirus infection
%A Austin B. Bigley
%A Emily C. LaVoy
%A Hawley Kunz
%A Justin Reed
%A Maryam Hussain
%A Mira Pistillo
%A Richard J. Simpson
%J Archive of "Physiological Reports".
%D 2017
%R 10.14814/phy2.13070
%X The magnitude of lymphocytosis following exercise is directly related to exercise intensity. Infection with cytomegalovirus (CMV) also augments lymphocytosis after exercise. It is not known if the enhanced T©\cell response to exercise due to CMV depends on exercise intensity. Furthermore, exercise©\induced changes in T©\cell expression of type I and type II cytokines are thought to be intensity dependent, but direct comparisons are lacking. The aim of this experiment was to determine if CMV affects the exercise©\induced redistribution of T©\cell subsets at varying intensities, and determine the effect of exercise intensity on CD8+ T©\cell cytokine expression. Seventeen cyclists (nine CMV seropositive; CMV+) completed three 30 min cycling trials at £¿5, +5, and +15% of blood lactate threshold (LT). T©\cell subsets in blood and intracellular expression of type I (IL©\2, interferon(IFN)©\¦Ã) and type II (IL©\4, IL©\10) cytokines by CD8+ T cells pre, post, and 1©\h post©\exercise were assessed by flow cytometry. Independently of CMV, T©\cell subset redistribution was greater after +15%LT compared to £¿5%LT (P < 0.05). Independently of intensity, CMV£¿ mobilized more low©\ (CD27+ CD28+) and medium©\ (CD27+ CD28£¿) differentiated T cells than CMV+, whereas CMV+ mobilized more high (CD27£¿ CD28£¿) differentiated T cells. The numbers of IL©\2+, IFN©\¦Ã+, IL©\4+, and IL©\10+ CD8+ T cells increased after exercise above LT. Only type I cytokine expression was influenced by exercise intensity (P < 0.05). In conclusion, T©\cell redeployment by exercise is directly related to exercise intensity, as are changes in the number of CD8+ T©\cells expressing type I cytokines. Although CMV+ mobilized more high©\differentiated T cells than CMV£¿, this occurred at all intensities. Therefore, the augmenting effect of CMV on T©\cell mobilization is independent of exercise intensity
%K Cytokine
%K infection
%K inflammation
%K lymphocyte
%K mobilization
%K physical activity
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5256156/